Research Service on ABC Transporters

Drug Screening with ABC Transporters (Substrate Specificity and Inhibition Studies)

  Testing of substrate specificity and inhibitory effects of compounds is offered as a research service, by means of “ATPase Assay” and “Membrane Vesicle Assay” using ABC transporter-expressing membranes from an insect Sf9 cell system. 
   The results are expected to be helpful for evaluation of drug disposition in various organs, including bile acid excretion, hepatotoxicity, and transport at the small intestine or blood-brain barrier.

Gene Expression Profiling
   It is very important for prediction of drug disposition in humans to determine expression levels of transporters in various tissues or cultured cells (“Expression Profiling”).
”Expression Profiling” by quantitative PCR is available as a research service for 47 kinds of human ABC transporters.

Analysis of Human MDR1 Polymorphism

 GenoMembrane undertakes polymorphism analysis of the human MDR1 (P-glycoprotein)-related genome area, covering the transcription-regulatory regions and the amino acid coding regions.
   GenoMembrane is exclusively licensed for polymorphism analysis of transcription-regulatory regions that influence the expression level of human MDR1.

<Reference relating to MDR1 polymorphism>
　Taniguchi S, Mochida Y, Uchiumi T, Tahira T, Hayashi K, Takagi K, Shimada M, Maehara Y, Kuwano H, Kono S, Nakano H, Kuwano M, Wada M. Genetic polymorphism at the 5' regulatory region of multidrug resistance 1 (MDR1) and its association with interindividual variation of expression level in the colon. Mol. Cancer Ther. 2003 2(12): 1351-1359.

   Please contact us for further details of these research services, including protocols, experimental period and cost.

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