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ABC Transporter ProductsGenoMembrane has excellent expertise and experience in the production of various drug transporters. We provide “ABC Transporter Membranes / Vesicles”, which are prepared from insect Sf9 cells expressing various ABC transporters. We also provide ready-to-use kit containing all reagents needed for ATPase assay using ABC Transporter Membranes and vesicular transport assay using ABC Transporter Vesicles. ATPase assay and vesiculare transport assay using our products are helpful to clarify the interactions between a compound of interest and ABC transporters.
GenoMembrane is licensed for exclusive right in Japan from Kyushu TLO Company, Limited to manufacture and sell the MRP2 products. (licensed patent; JP No.3695858)
(please click here for more details.) ABC Transporter Membranes /Vesicles
For ATPase AssayOne vial of the membrane producuts can perform 125 ATPase assays.
For Vesicular Transport Assay One vial of the membrane producuts can perform 50 vesicular transport assays.
*Because of basal transport activity of the Sf9 cell membrane and nonspecific adsorption of the labeled and non-labeled compound may be observed, depending upon the substrate used. Therefore, we recommend the use of Control (Cat.No. GM0003) as a negative control.
MDR1This products can be conducted for simple and convenient assay to determine the inhibitory activity of NMQ uptake against candidate compounds with fluorescent plate reader. For both ATPase Assay and Vesicular Transport Assay
* We changed the specifications of ABC Transporter products. Please find the attached file of correlating table. ⇒
Reagent Set for ABC Transporter Membrane/Vesicles
ABC Transporter ATPase Assay Reagent KitGenoMembrane provides “Ready-to-use” ATPase Assay kits. This kit can perform 160 ATPase assays and 32 phosphate standard (one kit full 96-well plate×2).
ABC Transporter ATPase Assay Reagents Kit (GM3020)
[Contents] ・Reaction Buffer ・100 mM Orthovanadate Solution ・200 mM MgATP Solution ・10 mM Phosphate Standard Solution ・100 mM Orthovanadate Solution ・Reductant ・Stop Solution ・Coloring A Solution ・Coloring B Solution
ABC Transporter Vesicular Reagent SetGenoMembrane provides two kinds of ready-to-use kit containing all reagents needed for vesicular transport assay using ABC Transporter Vesicles. This kit can perform 100 vesicular transport assays, which is sufficient for two GM vesicles products.
Vesicular Transport Assay Reagents Set
ABC Transporter and AssayABC TransportersThe ATP-Binding Cassette (ABC) transporters are a family of membrane proteins, members of which are found in various living organisms from microbes to humans. These transporters have ATPase activity and carry many drugs, fats, sterols and other endogenous metabolites. Forty-seven kinds of ABC transporter have already been identified in the human genome, and approximately 11 kinds are recognized as important drug transporters that transfer materials from the inside of cells to the outside. ATPase AssayABC transporter activity is driven by the energy derived from hydrolysis of ATP.
Membrane Vesicle AssayABC Transporter Vesicles, which have an inside-out vesicle structure, are prepared from membranes of insect Sf9 cells expressing ABC transporters for membrane vesicle assay. The transporters in these inside-out vesicles import the substrate from outside of the cell membrane vesicles to inside. Subsequently, quantitative measurement with HPLC or LC/MS/MS, or with radioactivity or fluorescence measurement in the case of appropriately labeled compounds, can be used to evaluate directly the transport activity by determining the amounts of the compounds in the cell membrane vesicles. |
Correlating table English.pdf(12.3KB)
