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ABC Transporter ProductsGenoMembrane has excellent expertise and experience in the production of various drug transporters. We provide gABC Transporter Membranes and Vesiclesh, which are prepared from insect Sf9 cells expressing various ABC transporters. We also provide ready-to-use kit containing all reagents needed for ATPase assay using ABC Transporter Membranes and vesicular transport assay using ABC Transporter Vesicles. ATPase assay and vesiculare transport assay using our products are helpful to clarify the interactions between a compound of interest and ABC transporters.
GenoMembrane is licensed for exclusive right in Japan from Kyushu TLO Company, Limited to manufacture and sell the MRP2 products. (licensed patent; JP No.3695858)
@(please click here for more details.) ABC Transporter Membranes and Reagent Kit for ATPase Assay
ABC Transporter Membranes One vial of the membrane producuts can perform 125 ATPase assays. The protocol and product size (2.5mg^0.5ml^vial) of all the following products is same.
Reagent Kit for ABC Transporter MembranesGenoMembrane provides gReady-to-useh ATPase Assay kits. This kit can perform 160 ATPase assays and 32 phosphate standard (one kit full 96-well plate~2).
ABC Transporter ATPase Assay Reagents Kit (GM3020)
[Contents]@EReaction Buffer E100 mM Orthovanadate Solution E200 mM MgATP Solution E10 mM Phosphate Standard Solution E100 mM Orthovanadate Solution EReductant EStop Solution EColoring A Solution EColoring B Solution
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ABC Transporter Vesicles and Reagent Kit for Vesicular Transport Assay
ABC Transporter Vesicles One vial of the membrane producuts can perform 50 vesicular transport assays.
Products Species Catalogue No. Leaflet Protocol Human Human Human Human Human
*Because of basal transport activity of the Sf9 cell membrane and nonspecific adsorption of the labeled and non-labeled compound may be observed, depending upon the substrate used. Therefore, we recommend the use of Control Vesicles (Cat.No. GM0003V) as a negative control.
Reagent Kit for ABC Transporter Vesicles New!
GenoMembrane provides two kinds of ready-to-use kit containing all reagents needed for vesicular transport assay using ABC Transporter Vesicles. This kit can perform 100 vesicular transport assays, which is sufficient for two GM vesicles products.
Vesicular Transport Assay Reagents Set
* We updated the protocol on Jan.13th, 2009. iGM3001:ver.2.0¨ver.2.1 GM3001:ver.2.1¨ver.2.2 j
ABC Transporter and AssayABC TransportersThe ATP-Binding Cassette (ABC) transporters are a family of membrane proteins, members of which are found in various living organisms from microbes to humans. These transporters have ATPase activity and carry many drugs, fats, sterols and other endogenous metabolites. Forty-seven kinds of ABC transporter have already been identified in the human genome, and approximately 11 kinds are recognized as important drug transporters that transfer materials from the inside of cells to the outside. ATPase AssayABC transporter activity is driven by the energy derived from hydrolysis of ATP.
Membrane Vesicle AssayABC Transporter Vesicles, which have an inside-out vesicle structure, are prepared from membranes of insect Sf9 cells expressing ABC transporters for membrane vesicle assay. The transporters in these inside-out vesicles import the substrate from outside of the cell membrane vesicles to inside. Subsequently, quantitative measurement with HPLC or LC/MS/MS, or with radioactivity or fluorescence measurement in the case of appropriately labeled compounds, can be used to evaluate directly the transport activity by determining the amounts of the compounds in the cell membrane vesicles.
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Human MDR1 M (ver.2.1)
